The Journal of Nutritional Biochemistry
Volume 10, Issue 11 , Pages 654-659 , November 1999

Allyl mercaptan, a major metabolite of garlic compounds, reduces cellular cholesterol synthesis and its secretion in Hep-G2 cells

  • Shanqin Xu

      Affiliations

    • Division of Nutritional Sciences, University of Illinois, Champaign, IL USA
    • ,
  • B.H Simon Cho

      Affiliations

    • Harlan E. Moore Heart Research Foundation, Champaign, IL USA
    • Corresponding Author InformationAddress correspondence to Dr. B.H.S. Cho, Division of Nutritional Sciences, University of Illinois, 503 S. Sixth Street, Champaign, IL USA 61820

Received 16 February 1999 ,Accepted 23 July 1999.

References 

  1. Jain RC. Effect of garlic on serum lipids, coagulability and fibrinolytic activity. Am. J. Clin. Nutr. 1977;30:1380–1381
  2. Bordia A. Effect of garlic on blood lipids in patients with coronary heart disease. Am. J. Clin. Nutr. 1981;34:2100–2103
  3. Augusti KT, Mathew PT. Lipid lowering effect of allicin (diallyl disulphide-oxide) on long term feeding to normal rats. Experientia. 1974;30:468–470
  4. Jain RC, Konar DB. Effect of garlic oil in experimental cholesterol atherosclerosis. Atherosclerosis. 1978;29:125–129
  5. Gebhardt R, Halgund B, Wagner KG. Inhibition of cholesterol biosynthesis by allicin and ajoene in rat hepatocytes and HepG2 cells. Biochim. Biophys. Acta. 1994;1213:57–62
  6. Yeh YY, Yeh SM. Garlic reduces plasma lipids by inhibiting hepatic cholesterol and triacylglycerol synthesis. Lipids. 1994;29:189–193
  7. Gebhardt R. Multiple inhibitory effects of garlic extracts on cholesterol biosynthesis in hepatocytes. Lipids. 1993;28:613–619
  8. Qureshi AA, Naji A, Zafeer ZD, Charles EE, Warren CB. Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic. Lipids. 1983;18:343–348
  9. Qureshi AA, Din ZZ, Abuirmeileh N, Burger WC, Ahmad Y, Elson CE. Suppression of avian hepatic lipid metabolism by solvent extracts of garlic (Impact on serum lipids). J. Nutr. 1983;113:1746–1755
  10. Lawson LD, Ransom DK, Huges BG. Inhibition of whole blood platelet aggregation by compounds in garlic clove extracts and commercial garlic products. Thromb. Res. 1992;65:141–156
  11. Lawson LD, Wang ZJ. Pre-hepatic fate of the organosulfur compounds derived from garlic (Allium sativum). Planta Med. 1993;59:A688
  12. Lawson LD. Bioactive organosulfur compounds of garlic products (Their role in reducing blood lipids). In:  Kinghorn AD,  Balandrin MF editor. Human Medicinal Agents From Plants. Washington, DC, USA: American Chemical Society; 1993;p. 306–330
  13. Egon-Schmind C, Eckardm R, Kemper EH. Metabolism of garlic constituents in the isolated perfused rat liver. Planta Med. 1992;58:301–305
  14. Gebhardt R, Beck H. Differential inhibitory effects of garlic-derived organosulfur compounds on cholesterol biosynthesis in primary rat hepatocyte cultures. Lipids. 1996;31:1269–1276
  15. Ellsworth JL, Erickson SK, Cooper AD. Very low and low density lipoprotein synthesis and secretion by the human hepatoma cell line Hep-G2 (Effects of free fatty acid). J. Lipid Res. 1986;27:858–874
  16. Dokko RC, Cho BHS, Chung BH. Cellular uptake of stearic, oleic, linoleic, and linolenic acid and their effects on synthesis and secretion of lipids in Hep-G2 cells. Int. J. Biochem. & Cell Biol. 1998;30:65–76
  17. Dashti N, Franklin FA, Abrahamson DR. Effect of ethanol on the synthesis and secretion of ApoA-1- and ApoB-containing lipoproteins in HepG2 cells. J. Lipid Res. 1996;37:810–824
  18. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951;193:267–275
  19. Folch T, Lee M, Stanlay GHS. A simple method for the isolation and purification of total lipids from animal tissue. J. Biol. Chem. 1957;226:497–509
  20. Yeh YY, Leveille GA. Effects of dietary protein on hepatic lipogenesis in the growing chick. J. Nutr. 1969;98:356–366
  21. Agarwal KC. Therapeutic actions of garlic constituents. Med. Res. Rev. 1996;16:111–124
  22. Scharfenberg K, Wagner R, Wagner KG. The cytotoxic effect of ajoene, a natural product from garlic, investigated with different cell lines. Cancer Lett. 1990;53:103–108
  23. Scharfenberg K, Ryll T, Wagner R, Wagner KG. Injuries to cultivated BJA-b cells by ajoene, a garlic-derived natural compound (Cell viability, glutathione metabolism, and pools of acidic amino acids). J. Cell Physiol. 1994;158:55–60
  24. Miccadei S, Nakae D, Kyle ME, Gilfor D, Farber JL. Oxidative cell injury in the killing of cultured hepatocytes by allyl alcohol. Arch. Biochem. Biophys. 1988;265:302–310
  25. Pinto JT, Qiao C, Xing J, Rivlin RS, Protomastro ML, Weissler ML, et al. Effects of garlic thioallyl derivatives on growth, glutathione concentration, and polyamine formation of human prostate carcinoma cells in culture. Am. J. Clin. Nutr. 1997;66:398–405
  26. Gebhardt R. Amplification of palmitate induced inhibition of cholesterol biosynthesis in cultured rat hepatocytes by garlic-derived organosulfur compounds. Phytomedicine. 1995;2:29–34
  27. Kumar RVO, Banerji A, Kurup CKR, Ramasarma T. The nature of inhibition of 3-hydroxy-3-methylglutaryl CoA reductase by garlic-derived diallyl disulfide. Biochim. Biophys. Acta. 1991;1078:219–225
  28. Goldfarb S, Pitot HC. Stimulatory effect of dietary lipid and cholestyramine on hepatic HMG CoA reductase. J. Lipid Res. 1972;13:797–801
  29. Goh EH, Heimberg M. Effect of oleic acid and cholesterol on the activity of hepatic hydroxymethylglutaryl coenzyme A reductase. FEBS Lett. 1976;63:209–210
  30. Goh EH, Heimberg M. Stimulation of hepatic cholesterol biosynthesis by oleic acid. Biochim. Biophys. Res. Co. 1973;55:382–388

☆ This work was supported by the fund for the Moore Investigator (BHSC) from the Harlan E. Moore Charitable Trust.

PII: S0955-2863(99)00056-X

The Journal of Nutritional Biochemistry
Volume 10, Issue 11 , Pages 654-659 , November 1999

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