The Journal of Nutritional Biochemistry
Volume 21, Issue 12 , Pages 1186-1192 , December 2010

Butyrate reduced lipopolysaccharide-mediated macrophage migration by suppression of Src enhancement and focal adhesion kinase activity

  • Ming-Chei Maa

      Affiliations

    • Institute of Molecular Systems Biomedicine, China Medical University, Taichung, Taiwan 40402, R.O.C.
    • Institute of Medical Science, China Medical University, Taichung, Taiwan 40402, R.O.C.
    • Corresponding Author InformationCorresponding authors. Ming-Chei Maa is to be contacted at Tel.: +886 4 2205 2121x7708; fax: +886 4 2205 3764. Tzeng-Horng Leu, Tel.: +886 6 235 3535x5468; fax: +886 6 274 9269.
    • ,
  • Miao Ying Chang

      Affiliations

    • Department of Medical Research, China Medical University Hospital, Taichung, Taiwan 40402, R.O.C.
    • ,
  • Ming-Yu Hsieh

      Affiliations

    • Institute of Molecular Systems Biomedicine, China Medical University, Taichung, Taiwan 40402, R.O.C.
    • ,
  • Yen-Jen Chen

      Affiliations

    • Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
    • Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
    • ,
  • Ching-Jau Yang

      Affiliations

    • Institute of Medical Science, China Medical University, Taichung, Taiwan 40402, R.O.C.
    • ,
  • Zuei-Ching Chen

      Affiliations

    • Institute of Medical Science, China Medical University, Taichung, Taiwan 40402, R.O.C.
    • ,
  • Yung Kuo Li

      Affiliations

    • Institute of Medical Science, China Medical University, Taichung, Taiwan 40402, R.O.C.
    • ,
  • Chia-Kuang Yen

      Affiliations

    • Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan 40402, R.O.C.
    • ,
  • Ruei-Ren Wu

      Affiliations

    • Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan 40402, R.O.C.
    • ,
  • Tzeng-Horng Leu

      Affiliations

    • Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
    • Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
    • Center for Gene Regulation and Signal Transduction Research, National Cheng Kung University, Tainan, Taiwan 70101, R.O.C.
    • Corresponding Author InformationCorresponding authors. Ming-Chei Maa is to be contacted at Tel.: +886 4 2205 2121x7708; fax: +886 4 2205 3764. Tzeng-Horng Leu, Tel.: +886 6 235 3535x5468; fax: +886 6 274 9269.

Received 8 July 2009 ,Revised 14 October 2009 ,Accepted 20 October 2009.

References 

  1. Leu TH, Maa MC. Functional implication of the interaction between EGF receptor and c-Src. Front Biosci. 2003;8:s28–s38
  2. Lowell CA, Berton G. Integrin signal transduction in myeloid leukocytes. J Leukoc Biol. 1999;65:313–320
  3. Meng F, Lowell CA. Lipopolysaccharide (LPS)-induced macrophage activation and signal transduction in the absence of Src-family kinases Hck, Fgr and Lyn. J Exp Med. 1997;185:1661–1670
  4. Maa MC, Chang MY, Chen YJ, Lin CH, Yu CJ, Yang YL, et al. Requirement of inducible nitric oxide synthase in lipopolysaccharide-mediated Src induction and macrophage migration. J Biol Chem. 2008;283:31408–31416
  5. Leu TH, Charoenfuprasert S, Yen CK, Fan CW, Maa MC. Lipopolysaccharide-induced c-Src expression plays a role in nitric oxide and TNFα secretion in macrophages. Mol Immunol. 2006;43:308–316
  6. Leu TH, Maa MC. Tyr-863 phosphorylation enhances focal adhesion kinase autophosphorylation. Oncogene. 2002;21:6992–7000
  7. Schlaepfer DD, Hauck CR, Sieg DJ. Signaling through focal adhesion kinase. Prog Biophys Mol Biol. 1999;71:435–478
  8. Owen KA, Pixley F, Thomas KS, Vicente-Manzanares M, Ray B, Horwitz AF, et al. Regulation of lamellipodial persistence, adhesion turnover, and motility in macrophages by focal adhesion kinase. J Cell Biol. 2007;179:1275–1287
  9. Augenlicht LH, Mariadason JM, Wilson A, Arango D, Yang W, Heerdt BG, et al. Short chain fatty acids and colon cancer. J Nutr. 2002;132:3804S–3808S
  10. Storer G, Trimble R, Illman RJ, Snoswell A, Topping D. Effects of dietary oat bran and diabetes on plasma and caecal volatile fatty acids in the rat. Nutr Res. 1983;3:519–526
  11. Hass R, Busche R, Luciano L, Reale E, Engelhardt WV. Lack of butyrate is associated with induction of Bax and subsequent apoptosis in the proximal colon of guinea pig. Gastroenterology. 1997;112:875–881
  12. Luciano L, Hass R, Busche R, von Engelhardt R, Reale E. Withdrawal of butyrate from the colonic mucosa triggers “mass apoptosis” primarily in the G0/G1 phase of the cell cycle. Cell Tissue Res. 1996;286:81–92
  13. Mandal M, Kumar R. Bcl-2 expression regulates sodium butyrate-induced apoptosis in human MCF-7 breast cancer cells. Cell Growth Differ. 1996;7:311–316
  14. Schwartz B, Avivi-Green C, Polak-Charcon S. Sodium butyrate induces retinoblastoma protein dephosphorylation, p16 expression and growth arrest of colon cancer cells. Mol Cell Biochem. 1998;188:21–30
  15. Lee JC, Maa MC, Yu HS, Wang JH, Yen CK, Wang ST, et al. Butyrate regulates the expression of c-Src and focal adhesion kinase and inhibits cell invasion of human colon cancer cells. Mol Carcinog. 2005;43:207–214
  16. Reddy BS. Nutritional factors and colon cancer. Crit Rev Food Sci Nutr. 1995;35:175–190
  17. Park JS, Lee EJ, Lee JC, Kim WK, Kim HS. Anti-inflammatory effects of short chain fatty acids in IFN-γ-stimulated RAW264.7 murine macrophage cells: involvement of NF-κB and ERK signaling pathways. Int Immunopharmacol. 2007;7:70–77
  18. Wang J, Mahmud SA, Bitterman PB, Huo Y, Slungaard A. Histone deacetylase inhibitors suppress TF-κB-dependent agonist-driven tissue factor expression in endothelial cells and monocytes. J Biol Chem. 2007;282:28408–28418
  19. Kanauchi O, Andoh A, Iwanaga T, Fujiyama Y, Mitsuyama K, Toyonaga A, et al. Germinated barley foodstuffs attenuate colonic mucosa damage and mucosal nuclear factor kappa B activity in a spontaneous colitis model. J Gastroenterol Hepatol. 1999;14:1173–1179
  20. Butzner JD, Parmar R, Bell CJ, Dalal V. Butyrate enema therapy stimulates mucosa repair in experimental colitis in the rat. Gut. 1996;38:568–573
  21. Chen JS, Faller DV, Spanjaard RA. Short-chain fatty acid inhibitors of histone deacetylases: promising anticancer therapeutics?. Curr Cancer Drug Targets. 2003;3:219–236
  22. Bradburn DM, Mathers JC, Gunn A, Burn J, Chapman PD, Johnston IDA. Colonic fermentation of complex carbohydrates in patients with familial adenomatous polyposis. Gut. 1993;34:630–636
  23. Barshishat M, Polak-Charcon S, Schwartz B. Butyrate regulates E-cadherin transcription, isoform expression and intracellular position in colon cancer cells. Br J Cancer. 2000;82:195–203
  24. Souleimani A, Asselin C. Regulation of c-myc expression by sodium butyrate in the colon carcinoma cell line Caco-2. FEBS Lett. 1993;326:45–50
  25. Archer S, Meng S, Hodin RA. p21(WAF1) is required for butyrate-mediated growth inhibition of human colon cancer cells. Proc Natl Acad Sci U S A. 1998;95:6791–6796
  26. Kostyniuk CL, Dehm SM, Batten D, Bonham K. The ubiquitous and tissue specific promoters of the human SRC gene are repressed by inhibitors of histone deacetylases. Oncogene. 2002;21:6340–6347
  27. Maa MC, Leu TH. Vanadate-dependent FAK activation is accomplished by the sustained FAK Tyr-576/577 phosphorylation. Biochem Biophys Res Commun. 1998;251:344–349
  28. Tanno T, Fujiwara A, Sakaguchi K, Tanaka K, Takenaka S, Tsuyama S. Slit3 regulates cell motility through Rac/Cdc42 activation in lipopolysaccharide-stimulated macrophages. FEBS Lett. 2007;581:1022–1026
  29. Yoshida M, Horinouchi S, Beppu T. Trichostatin A and trapoxin: novel chemical probes for the role of histone acetylation in chromatin structure and function. Bioessays. 1995;17:423–430
  30. Dokmanovic M, Clarke C, Marks PA. Histone deacetylase inhibitors: overview and perspectives. Mol Cancer Res. 2007;5:981–989
  31. Park YM, Febbraio M, Silverstein RL. CD36 modulates migration of mouse and human macrophages in response to oxidized LDL and may contribute to macrophage trapping in the arterial intima. J Clin Invest. 2009;119:136–145
  32. Bolden JE, Peart MJ, Johnstone RW. Anticancer activities of histone deacetylase inhibitors. Nat Rev Drug Disc. 2006;5:769–784
  33. Bhalla KN. Epigenetic and chromatin modifiers as targeted therapy of hematologic malignancies. J Clinc Oncol. 2005;23:3971–3993
  34. Chakravotty D, Koide N, Kato Y, Sugiyama T, Mu MM, Yoshida T, et al. The inhibitory action of butyrate on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells. J Endotoxin Res. 2000;6:243–247
  35. Calao M, Burny A, Quivy V, Dekoninck A, Van Lint C. A pervasive role of histone acetyltransferases and deacetylases in an NF-κB signaling code. Trends Biochem Sci. 2008;33:339–349

☆ This work was supported by National Science Council grants to M.-C.M (NSC98-2311-B-039-002-MY3) and T.-H.L (NSC97-2320-B-006-024-MY3). Additional support came from NHRI (NHRI-EX-98-9828BI) and China Medical University (CMU97-103 and CMU97-193) to T.-H.L. and M.-C.M., respectively.

PII: S0955-2863(09)00218-6

doi: 10.1016/j.jnutbio.2009.10.004

The Journal of Nutritional Biochemistry
Volume 21, Issue 12 , Pages 1186-1192 , December 2010

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