The Journal of Nutritional Biochemistry
Volume 21, Issue 9 , Pages 881-886 , September 2010

Evaluation of cardiovascular risk and oxidative stress parameters in hypercholesterolemic subjects on a standard healthy diet including low-fat milk enriched with plant sterols

  • Celia Bañuls

      Affiliations

    • Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain
    • CIBER CB/06/02/0045 Research Group, CIBER Actions in Epidemiology and Public Health, Valencia, Spain
    • ,
  • María L. Martínez-Triguero

      Affiliations

    • Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain
    • ,
  • Antonio López-Ruiz

      Affiliations

    • Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain
    • ,
  • Carlos Morillas

      Affiliations

    • Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain
    • ,
  • Ramon Lacomba

      Affiliations

    • Nutrition and Food Chemistry, Faculty of Pharmacy, University of Valencia, Valencia, Spain
    • ,
  • Víctor M. Víctor

      Affiliations

    • Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain
    • Dr. Peset Hospital Research Foundation, Valencia, Spain
    • ,
  • Milagros Rocha

      Affiliations

    • Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain
    • Dr. Peset Hospital Research Foundation, Valencia, Spain
    • ,
  • Antonio Hernández-Mijares

      Affiliations

    • Service of Endocrinology, University Hospital Dr. Peset, Valencia, Spain
    • CIBER CB/06/02/0045 Research Group, CIBER Actions in Epidemiology and Public Health, Valencia, Spain
    • Dr. Peset Hospital Research Foundation, Valencia, Spain
    • Department of Medicine, Faculty of Medicine, University of Valencia, Valencia, Spain
    • Corresponding Author InformationCorresponding author. Service of Endocrinology, University Hospital Dr. Peset, 46017 Valencia, Spain.

Received 22 December 2008 ,Revised 26 June 2009 ,Accepted 1 July 2009.

References 

  1. Expert Panel on Detection, Evaluation and Treatment of High Blood Cholesterol in Adults . Executive summary of the Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). JAMA. 2001;285:2486–2497
  2. Hunninghake DB, Stein EA, Dujovne CA, et al. The efficacy of intensive dietary therapy alone or combined with lovastatin in outpatients with hypercholesterolemia. N Engl J Med. 1993;328:1213–1219
  3. Davidson MH, Kong JC, Drennan KB, Story K, Anderson GH. Efficacy of the National Cholesterol Education Program Step I diet. A randomized trial incorporating quick-service foods. Arch Intern Med. 1996;156:305–312
  4. Katan MB, Grundy SM, Jones P, Law M, Miettinen T, Paoletti R. Efficacy and safety of plant stanols and sterols in the management of blood cholesterol levels. Mayo Clin Proc. 2003;78:965–978
  5. von Bergmann K, Sudhop T, Lutjohann D. Cholesterol and plant sterol absorption: recent insights. Am J Cardiol. 2005;96:10D–14D
  6. Noakes M, Clifton PM, Doornbos AME, Trautwein EA. Plant sterol ester-enriched milk and yoghurt effectively reduce serum cholesterol in modestly hypercholesterolemic subjects. Eur J Nutr. 2005;44:214–222
  7. Seppo L, Jauhiainen T, Nevala R, Poussa T, Korpela R. Plant stanol esters in low-fat milk products lower serum total and LDL cholesterol. Eur J Nutr. 2007;46:111–117
  8. Jiménez-Escrig A, Santos-Hidalgo AB, Saura-Calixto F. Common sources and estimated intake of plant sterols in the Spanish diet. J Agric Food Chem. 2006;54:3462–3471
  9. Cleghorn CL, Skeaff CM, Mann J, Chisholm A. Plant sterol-enriched spread enhances the cholesterol-lowering potential of a fat-reduced diet. Eur J Clin Nutr. 2003;57:170–176
  10. Clifton PM, Noakes M, Sullivan D, et al. Cholesterol-lowering effects of plant sterol esters differ in milk, yoghurt, bread and cereal. Eur J Clin Nutr. 2004;58:503–509
  11. Thomsen AB, Hansen HB, Christiansen C, Green H, Berger A. Effect of free plant sterols in low-fat milk on serum lipid profile in hypercholesterolemic subjects. Eur J Clin Nutr. 2004;58:860–870
  12. Lloyd-Jones DM, Liu K, Tian L, Greenland P. Narrative review: Assessment of C-reactive protein in risk prediction for cardiovascular disease. Ann Intern Med. 2006;145:35–42
  13. Ridker PM, Rifai N, Cook NR, Bradwin G, Buring JE. Non-HDL cholesterol, apolipoproteins A-I and B100, standard lipid measures, lipid ratios, and CRP as risk factors for cardiovascular disease in women. J Am Med Assoc. 2005;294:326–333
  14. AbuMweis SS, Vanstone CA, Ebine N, et al. Intake of a single morning dose of standard and novel plant sterol preparations for 4 weeks does not dramatically affect plasma lipid concentrations in humans. J Nutr. 2006;136:1012–1016
  15. Hansel B, Nicolle C, Lalanne F, Tondu F, Lassel T, Donazzolo Y. Effect of low-fat, fermented milk enriched with plant sterols on serum lipid profile and oxidative stress in moderate hypercholesterolemia. Am J Clin Nutr. 2007;86:790–796
  16. Madsen MB, Jensen AM, Schmidt EB. The effect of a combination of plant sterol-enriched foods in mildly hypercholesterolemic subjects. Clin Nutr. 2007;26:792–798
  17. De Jong A, Plat J, Bast A, Godschalk RW, Basu S, Mensink RP. Effects of plant sterol and stanol ester consumption on lipid metabolism, antioxidant status and markers of oxidative stress, endothelial function and low-grade inflammation in patients on current statin treatment. Eur J Clin Nutr. 2008;62:263–273
  18. Law M. Plant sterol and stanol margarines and health. Br Med J. 2000;320:861–864
  19. Colgan HA, Floyd S, Noone EJ, Gibney MJ, Roche HM. Increased intake of fruit and vegetables and a low-fat diet, with and without low-fat plant sterol-enriched spread consumption: effects on plasma lipoprotein and carotenoid metabolism. J Hum Nutr Diet. 2004;17:561–569
  20. Plana N, Nicolle C, Ferre R, et al. Plant sterol-enriched fermented milk enhances the attainment of LDL-cholesterol goal in hypercholesterolemic subjects. Eur J Nutr. 2008;47:32–39
  21. Mataix J, Mañas M, Llopis J, Martínez de Victoria E. Tablas de composición de alimentos españoles. 4th ed.. Granada: Universidad de Granada; 2003;
  22. Allain CC, Poon LS, Chan CSG, et al. Enzymatic determination of total serum cholesterol. Clin Chem. 1974;20:470–475
  23. Bucolo G, David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem. 1973;19:476–482
  24. Sugiuchi H, Uji Y, Okabe H, et al. Direct measurement of high-density lipoprotein cholesterol in serum with polyethylene glycol-modified enzymes and sulfated alphacyclodextrin. Clin Chem. 1995;41:717–723
  25. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of preparative ultracentrifuge. Clin Chem. 1972;18:499–502
  26. Esterbauer H, Striegl G, Puhl H, Rotheneder M. Continuous monitoring of in vitro oxidation of human low density lipoprotein. Free Radic Res Commun. 1989;6:67–75
  27. Miller NJ, Rice Evans C, Davies MJ, et al. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci Colch. 1993;84:407–412
  28. Olmedilla B, Granado F, Rojas-Hidalgo E, Blanco I. A rapid separation of ten carotenoids, three retinoids, alpha-tocopherol and d-alpha-tocopherol acetate by high performance liquid chromatography and its application to serum and vegetable samples. J Liq Chrom. 1990;13:1455–1483
  29. Yagi K. Simple assay for the level of total lipid peroxides in serum or plasma. Methods Mol Biol. 1998;108:101–106
  30. Bae CY, Keenan JM, Wenz J, McCaffrey DJ. A clinical trial of the American Heart Association step one diet for treatment of hypercholesterolemia. J Fam Pract. 1991;33:249–254
  31. De Backer G, Ambrosioni E, Borch-Johnsen K, American College of Cardiology , American Heart Association , European Society of Cardiology . European guidelines on cardiovascular disease prevention in clinical practice. Third Joint Task Force of European and other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of eight societies and by invited experts). Atherosclerosis. 2004;173:381–391
  32. Chan YM, Demonty I, Pelled D, Jones PJ. Olive oil containing olive oil fatty acid esters of plant sterols and dietary diacylglycerol reduces low-density lipoprotein cholesterol and decreases the tendency for peroxidation in hypercholesterolaemic subjects. Br J Nutr. 2007;98:563–570
  33. Erlinger TP, Miller ER, Charleston J, Appel LJ. Inflammation modifies the effects of a reduced-fat low-cholesterol diet on lipids: results from the DASH-sodium trial. Circulation. 2003;108:150–154
  34. Tur-Mari J, Sureda A, Pons A. Blood cells as functional markers of antioxidant vitamin status. Br J Nutr. 2006;96:S38–S41
  35. Plat J, van Onselen EN, van Heugten MM, Mensink RP. Effects on serum lipids, lipoproteins and fat soluble antioxidant concentrations of consumption frequency of margarines and shortenings enriched with plant stanol esters. Eur J Clin Nutr. 2000;54:671–677
  36. Granado F, Olmedilla B, Blanco I, Rojas-Hidalgo E. Major fruit and vegetable contributors to the main serum carotenoids in the Spanish diet. Eur J Clin Nutr. 1996;50:246–250
  37. Judd JT, Baer DJ, Chen SC, Clevidence BA, Muesing RA, Kramer M, et al. Plant sterol esters lower plasma lipids and most carotenoids in mildly hypercholesterolemic adults. Lipids. 2002;37:33–42
  38. Sanchez-Muniz FJ, Maki KC, Schaefer EJ, Ordovas JM. Serum lipid and antioxidant responses in hypercholesterolemic men and women receiving plant sterol esters vary by apolipoprotein E genotype. J Nutr. 2009;139:13–19
  39. Noakes M, Clifton P, Ntanios F, Shrapnel W, Record I, McInerney J. An increase in dietary carotenoids when consuming plant sterols or stanols is effective in maintaining plasma carotenoid concentrations. Am J Clin Nutr. 2002;75:79–86
  40. Victor VM, Rocha M. Targeting antioxidants to mitochondria: a potential new therapeutic strategy for cardiovascular diseases. Curr Pharm Des. 2007;13:845–863
  41. D'Odorico A, Martines D, Kiechl S, Egger G, Oberhollenzer F, Bonvicini P, et al. High plasma levels of alpha- and beta-carotene are associated with a lower risk of atherosclerosis: results from the Bruneck study. Atherosclerosis. 2000;153:231–239
  42. Korpela R, Seppo L, Laakso J, et al. Dietary habits affect the susceptibility of low-density lipoprotein to oxidation. Eur J Clin Nutr. 1999;53:802–807
  43. Schwab US, Vogel S, Lammi-Keefe CJ, et al. Varying dietary fat type of reduced-fat diets has little effect on the susceptibility of LDL to oxidative modification in moderately hypercholesterolemic subjects. J Nutr. 1998;128:1703–1709
  44. Shrestha S, Freake HC, McGrane MM, Volek JS, Fernandez ML. A combination of psyllium and plant sterols alters lipoprotein metabolism in hypercholesterolemic subjects by modifying the intravascular processing of lipoproteins and increasing LDL uptake. J Nutr. 2007;137:1165–1170
  45. Brufau G, Quílez J, Angel Canela M, Salas-Salvadó J, Bulló MM, Rafecas M. Evaluation of lipid oxidation after ingestion of bakery products enriched with phytosterols, beta-carotene and alpha-tocopherol. Clin Nutr. 2004;23:1390–1397
  46. Mannarino E, Pirro M, Cortese C, et al. Effects of a phytosterol-enriched dairy product on lipids, sterols and 8-isoprostane in hypercholesterolemic patients: a multicenter Italian study. Nutr Metab Cardiovasc Dis. 2009;19:84–90

☆ All authors have approved the final version of the manuscript. None of the authors has any personal or financial conflict of interest.

PII: S0955-2863(09)00151-X

doi: 10.1016/j.jnutbio.2009.07.001

The Journal of Nutritional Biochemistry
Volume 21, Issue 9 , Pages 881-886 , September 2010

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