The Journal of Nutritional Biochemistry
Volume 20, Issue 12 , Pages 982-991 , December 2009

Effects of chromium picolinate on glucose uptake in insulin-resistant 3T3-L1 adipocytes involve activation of p38 MAPK

Received 19 March 2008 ,Revised 1 September 2008 ,Accepted 5 September 2008.

References 

  1. Goldstein BJ. Insulin resistance as the core defect in type 2 diabetes mellitus. Am J Cordiol. 2002;90:3G–10G
  2. Groop LC. Insulin resistance: the fundamental trigger of type 2 diabetes. Diabetes Obes Metab. 1999;1:S1–S7
  3. Petersen KF, Shulman GI. Pathogenesis of skeletal muscle insulin resistance in type 2 diabetes mellitus. Am J Cardiol. 2002;90:11G–18G
  4. Rattarasarn C. Physiological and pathophysiological regulation of regional adipose tissue in the development of insulin resistance and type 2 diabetes. Acta Physiol (Oxf). 2006;186:87–101
  5. Toledo FG, Sniderman AD, Kelley DE. Influence of hepatic steatosis (fatty liver) on severity and composition of dyslipidemia in type 2 diabetes. Diab Care. 2006;29:1845–1850
  6. Henry RR. Insulin resistance: from predisposing factor to therapeutic target in type 2 diabetes. Clin Ther. 2003;25:B47–B63
  7. McCarty MF. Nutraceutical resources for diabetes prevention—an update. Med Hypotheses. 2005;64:151–158
  8. Anderson RA. Chromium in the prevention and control of diabetes. Diabetes Metab. 2000;26:22–27
  9. Sahin K, Onderci M, Tuzcu M, Ustundag B, Cikim G, Ozercan IH, et al. Effect of chromium on carbohydrate and lipid metabolism in a rat model of type 2 diabetes mellitus: the fat-fed, streptozotocin-treated rat. Metabolism. 2007;56:1233–1240
  10. Vladeva SV, Terzieva DD, Arabadjiiska DT. Effect of chromium on the insulin resistance in patients with type II diabetes mellitus. Folia Med (Plovdiv). 2005;47:59–62
  11. Schwarz K, Mertz W. Chromium (III) and the glucose tolerance factor. Arch Biochem Biophys. 1959;85:292–295
  12. Jeejeebhoy KN, Chu RC, Marliss EB, Greenberg GR, Bruce-Robertson A. Chromium deficiency, glucose intolerance, and neuropathy reversed by chromium supplementation in a patient receiving long-term total parenteral nutrition. Am J Clin Nutr. 1977;30:531–538
  13. Balk EM, Tatsioni A, Lichtenstein AH, Lau J, Pittas AG. Effect of chromium supplementation on glucose metabolism and lipids: a systematic review of randomized controlled trials. Diabetes Care. 2007;30:2154–2163
  14. Kim DS, Kim TW, Park IK, Kang JS, Om AS. Effects of chromium picolinate supplementation on insulin sensitivity, serum lipids, and body weight in dexamethasone-treated rats. Metabolism. 2002;51:589–594
  15. Morris BW, Kouta S, R Robinson R, MacNeil S, Heller S. Chromium supplementation improves insulin resistance in patients with type 2 diabetes mellitus. Diabet Med. 2000;17:684–685
  16. Vincent JB. Mechanisms of chromium action: low-molecular-weight chromium-binding substance. J Am Coll Nutr. 1999;18:6–12
  17. Vincent JB. Elucidating a biological role for chromium at a molecular level. Acc Chem Res. 2000;33:503–510
  18. Costa M. Toxicity and carcinogenicity of Cr(VI) in animal models and humans. Crit Rev Toxicol. 1997;27:431–442
  19. Becker DS, Long ER, Proctor DM, Ginn TC. Evaluation of potential toxicity and bioavailability of chromium in sediments associated with chromite ore processing residue. Environ Toxicol Chem. 2006;25:2576–2583
  20. Brautigan DL, Kruszewski A, Wang H. Chromium and vanadate combination increases insulin-induced glucose uptake by 3T3-L1 adipocytes. Biochem Biophys Res Commun. 2006;347:769–773
  21. Wang H, Kruszewski A, Brautiqun DL. Cellular chromium enhances activation of insulin receptor kinase. Biochemistry. 2005;44:8167–8175
  22. Wang ZQ, Zhang XH, Russell JC, Hulver M, Cefalu WT. Chromium picolinate enhances skeletal muscle cellular insulin signaling in vivo in obese, insulin-resistant JCR:LA-cp rats. J Nutr. 2006;136:415–420
  23. Chen G, Liu P, Patter GR, Tackett L, Bhonaqiri P, Strawbridge AB, et al. Chromium activates GLUT4 trafficking and enhances insulin-stimulated glucose transport in 3T3-L1 adipocytes via a cholesterol-dependent mechanism. Mol Endocrinol. 2006;20:857–870
  24. Thomson MJ, Williams MG, Frost SC. Development of insulin resistance in 3T3-L1 adipocytes. J Biol Chem. 1997;272:7759–7764
  25. Chiang SH, Baumann CA, Kanzaki M, Thurmond DC, Watson RT, Neudauer CL, et al. Insulin-stimulated GLUT4 translocation requires the CAP-dependent activation of TC10. Nature. 2001;410:944–948
  26. Sweeney G, Somwar R, Ramlal T, Volchuk A, Ueyama A, Klip A. An inhibitor of p38 mitogen-activated protein kinase prevents insulin-stimulated glucose transport but not glucose transporter translocation in 3T3-L1 adipocytes and L6 myotubes. J Biol Chem. 1999;274:10071–10078
  27. Anderson RA, Cheng N, Bryden NA, Polansky MM, Cheng N, Chi J, et al. Elevated intakes of supplemental chromium improve glucose and insulin variables in individuals with type 2 diabetes. Diabetes. 1997;46:1786–1791
  28. Shinde UA, Sharma G, Xu YJ, Dhalla NS, Goyal RK. Anti-diabetic activity and mechanism of action of chromium chloride. Exp Clin Endocrinol Diabetes. 2004;112:248–252
  29. Shindea UA, Sharma G, Xu YJ, Dhalla NS, Goyal RK. Insulin sensitising action of chromium picolinate in various experimental models of diabetes mellitus. J Trace Elem Med Biol. 2004;18:23–32
  30. Ross SA, Chen X, Hope HR, Sun S, McMahon EG, Broschat K, et al. Development and comparison of two 3T3-L1 adipocyte models of insulin resistance: increased glucose flux vs glucosamine treatment. Biochem Biophys Res Commun. 2000;273:1033–1041
  31. Khan AH, Pessin JE. Insulin regulation of glucose uptake: a complex interplay of intracellular signalling pathways. Diabetologia. 2002;45:1475–1483
  32. Saltiel AR, Kahn CR. Insulin signalling and the regulation of glucose and lipid metabolism. Nature. 2001;414:799–806
  33. Cusi K, Maezono K, Osman A, Pendergrass M, Patti ME, Pratipanawatr T, et al. Insulin resistance differentially affects the PI 3-kinase-and MAP kinase-mediated signaling in human muscle. J Clin Invest. 2000;105:311–320
  34. Koistinen HA, Chibalin AV, Zierath JR. Aberrant p38 mitogen-activated protein kinase signalling in skeletal muscle from Type 2 diabetic patients. Diabetologia. 2003;46:1324–1328
  35. Montessuit C, Rosenblatt-Velin N, Papageorgiou I, Campos L, Pellieux C, Palma T, et al. Regulation of glucose transporter expression in cardiac myocytes: p38 MAPK is a strong inducer of GLUT4. Cardiovasc Res. 2004;64:94–104
  36. Somwar R, Perreault M, Kapur S, Taha C, Sweeney G, Ramlal T, et al. Activation of p38 mitogen-activated protein kinase alpha and beta by insulin and contraction in rat skeletal muscle: potential role in the stimulation of glucose transport. Diabetes. 2000;49:1794–1800
  37. Bazuine M, Carlotti F, Rabelink MJ, Vellinga J, Hoeben RC, Maassen JA. The p38 MAPK-inhibitor SB203580 reduces glucose turnover by the GLUT4 transporter of 3T3-L1 adipocytes in the insulin-stimulated state. Endocrinology. 2005;146:1818–1824
  38. Kumar N, Dey CS. Metformin enhances insulin signalling in insulin-dependent and -independent pathways in insulin resistant muscle cells. Br J Pharmacol. 2002;137:329–336
  39. Bazuine M, Ouwens DM, Gomes de Mesquita DS, Maassen JA. Arsenite stimulated glucose transport in 3T3-L1 adipocytes involves both Glut4 translocation and p38 MAPK activity. Eur J Biochem. 2003;270:3891–3903
  40. Samet JM, Graves LM, Quay J, Dailey LA, Devlin RB, Ghio AJ, et al. Activation of MAPKs in human bronchial epithelial cells exposed to metals. Am J Physiol. 1998;275:L551–L558
  41. Somwar R, Koterski S, Sweeney G, Sciotti R, Djuric S, Berg C, et al. A dominant-negative p38 MAPK mutant and novel selective inhibitors of p38 MAPK reduce insulin-stimulated glucose uptake in 3T3-L1 adipocytes without affecting GLUT4 translocation. J Biol Chem. 2002;277:50386–50395
  42. Somwar R, Kim DY, Sweeney G, Huang C, Niu W, Lador C, et al. GLUT4 translocation precedes the stimulation of glucose uptake by insulin in muscle cells: potential activation of GLUT4 via p38 mitogen-activated protein kinase. Biochem J. 2001;359:639–649

 This study was supported by a grant (No. C13-101) from the Scientific Innovation Program of Fudan University and was also partially supported by a grant-in-aid from Shanghai Leading Academic Discipline Project (B119).

PII: S0955-2863(08)00202-7

doi: 10.1016/j.jnutbio.2008.09.002

The Journal of Nutritional Biochemistry
Volume 20, Issue 12 , Pages 982-991 , December 2009