The Journal of Nutritional Biochemistry
Volume 20, Issue 10 , Pages 816-822 , October 2009

Green and black tea extracts inhibit HMG-CoA reductase and activate AMP kinase to decrease cholesterol synthesis in hepatoma cells

  • Dev K. Singh

      Affiliations

    • Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA
    • ,
  • Subhashis Banerjee

      Affiliations

    • Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
    • ,
  • Todd D. Porter

      Affiliations

    • Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA
    • Graduate Center for Toxicology, University of Kentucky, Lexington, KY 40536, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1 859 257 1137.

Received 15 May 2008 ,Revised 24 July 2008 ,Accepted 29 July 2008.

References 

  1. Maron DJ, Lu GP, Cai NS, Wu ZG, Li YH, Chen HA, et al. Cholesterol-lowering effect of a theaflavin-enriched green tea extract: a randomized controlled trial. Arch Intern Med. 2003;163:1448–1453
  2. Davies MJ, Judd JT, Baer DJ, Clevidence BA, Paul DR, Edwards AJ, et al. Black tea consumption reduces total and LDL cholesterol in mildly hypercholesterolemic adults. J Nutr. 2003;133:3298S–3302S
  3. Koo SI, Noh SK. Green tea as inhibitor of the intestinal absorption of lipids: potential mechanism for its lipid-lowering effect. J Nutr Biochem. 2007;18:179–183
  4. Bursill CA, Abbey M, Roach PD. A green tea extract lowers plasma cholesterol by inhibiting cholesterol synthesis and upregulating the LDL receptor in the cholesterol-fed rabbit. Atherosclerosis. 2007;193:86–93
  5. Bursill CA, Roach PD. A green tea catechin extract upregulates the hepatic low-density lipoprotein receptor in rats. Lipids. 2007;42:621–627
  6. Chan PT, Fong WP, Cheung YL, Huang Y, Ho WK, Chen ZY. Jasmine green tea epicatechins are hypolipidemic in hamsters (Mesocricetus auratus) fed a high fat diet. J Nutr. 1999;129:1094–1101
  7. Gebhardt R. Variable influence of kaempferol and myricetin on in vitro hepatocellular cholesterol biosynthesis. Planta Med. 2003;69:1071–1074
  8. Gebhardt R. Inhibition of cholesterol biosynthesis in primary cultured rat hepatocytes by artichoke (Cynara scolymus L.) extracts. J Pharmacol Exp Ther. 1998;286:1122–1128
  9. Abe I, Seki T, Umehara K, Miyase T, Noguchi H, Sakakibara J, et al. Green tea polyphenols: novel and potent inhibitors of squalene epoxidase. Biochem Biophys Res Commun. 2000;268:767–771
  10. Laden BP, Porter TD. Resveratrol inhibits human squalene monooxygenase. Nutr Res. 2001;21:747–753
  11. Bursill C, Roach PD, Bottema CD, Pal S. Green tea upregulates the low-density lipoprotein receptor through the sterol-regulated element binding protein in HepG2 liver cells. J Agric Food Chem. 2001;49:5639–5645
  12. Bursill CA, Roach PD. Modulation of cholesterol metabolism by the green tea polyphenol (−)-epigallocatechin gallate in cultured human liver (HepG2) cells. J Agric Food Chem. 2006;54:1621–1626
  13. Espenshade PJ, Hughes AL. Regulation of sterol synthesis in eukaryotes. Annu Rev Genet. 2007;41:401–427
  14. Beg ZH, Stonik JA, Brewer HB. Modulation of the enzymic activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase by multiple kinase systems involving reversible phosphorylation: a review. Metabolism. 1987;36:900–917
  15. Carling D, Clarke PR, Zammit VA, Hardie DG. Purification and characterization of the AMP-activated protein kinase. Copurification of acetyl-CoA carboxylase kinase and 3-hydroxy-3-methylglutaryl-CoA reductase kinase activities. Eur J Biochem. 1989;186:129–136
  16. Hardie DG. Minireview: the AMP-activated protein kinase cascade: the key sensor of cellular energy status. Endocrinology. 2003;144:5179–5183
  17. Towler MC, Hardie DG. AMP-activated protein kinase in metabolic control and insulin signaling. Circ Res. 2007;100:328–341
  18. Brown MS, Goldstein JL. Suppression of 3-hydroxy-3-methylglutaryl coenzyme A reductase activity and inhibition of growth of human fibroblasts by 7-ketocholesterol. J Biol Chem. 1974;249:7306–7314
  19. Zhou G, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, et al. Role of AMP-activated protein kinase in mechanism of metformin action. J Clin Invest. 2001;108:1167–1174
  20. Beg ZH, Stonik JA, Brewer HB. 3-Hydroxy-3-methylglutaryl coenzyme A reductase: regulation of enzymatic activity by phosphorylation and dephosphorylation. Proc Natl Acad Sci U S A. 1978;75:3678–3682
  21. Zang M, Xu S, Maitland-Toolan KA, Zuccollo A, Hou X, Jiang B, et al. Polyphenols stimulate AMP-activated protein kinase, lower lipids, and inhibit accelerated atherosclerosis in diabetic LDL receptor-deficient mice. Diabetes. 2006;55:2180–2191

☆ Supported in part by grant AT-003488 from the National Institutes of Health.

PII: S0955-2863(08)00173-3

doi: 10.1016/j.jnutbio.2008.07.011

The Journal of Nutritional Biochemistry
Volume 20, Issue 10 , Pages 816-822 , October 2009

Article Tools

* Image Usage & Resolution