Grape-seed procyanidins prevent low-grade inflammation by modulating cytokine expression in rats fed a high-fat diet

Diet effect on CRP expression in liver. Liver mRNA was extracted, corresponding cDNA was synthesized and CRP gene expression was measured by quantitative real-time RT-PCR. Student's t test was used to evaluate significance between groups (P<.05).

Diet effect on gene expression in mesenteric adipose tissue. mRNA was extracted, corresponding cDNA was synthesized and CRP, IL-6, adiponectin and TNF-α gene expression was measured by quantitative real-time RT-PCR. ANOVA test was used to evaluate significance between groups (P<.05).