The Journal of Nutritional Biochemistry
Volume 18, Issue 10 , Pages 693-699 , October 2007

Red blood cell folate vitamer distribution in healthy subjects is determined by the methylenetetrahydrofolate reductase C677T polymorphism and by the total folate status

  • Yvo M. Smulders

      Affiliations

    • Department of Internal Medicine and Institute for Cardiovascular Research ICaR-VU, VU University Medical Center, Amsterdam 1007 MB, The Netherlands
    • Corresponding Author InformationCorresponding author. Tel.: +31 20 4444307; fax: +31 20 4444313.
    • ,
  • Desiree E.C. Smith

      Affiliations

    • Department of Clinical Chemistry, VU University Medical Center, Amsterdam 1007 MB, The Netherlands
    • ,
  • Robert M. Kok

      Affiliations

    • Department of Clinical Chemistry, VU University Medical Center, Amsterdam 1007 MB, The Netherlands
    • ,
  • Tom Teerlink

      Affiliations

    • Department of Clinical Chemistry, VU University Medical Center, Amsterdam 1007 MB, The Netherlands
    • ,
  • Henkjan Gellekink

      Affiliations

    • Laboratory of Pediatrics and Neurology, Radboud University Nijmegen Medical Center, Nijmegen 6500 HB, The Netherlands
    • ,
  • Wouter H.J. Vaes

      Affiliations

    • BU Analytical Research, TNO Quality of Life, Zeist 3700 AJ, The Netherlands
    • ,
  • Coen D.A. Stehouwer

      Affiliations

    • Department of Internal Medicine, University Hospital Maastricht and Maastricht University, Maastricht 6202 AZ, The Netherlands
    • ,
  • Cornelis Jakobs

      Affiliations

    • Department of Clinical Chemistry, VU University Medical Center, Amsterdam 1007 MB, The Netherlands

Received 7 August 2006 ,Revised 9 November 2006 ,Accepted 9 November 2006.

References 

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  9. Friso S, Choi SW, Girelli D, Mason JB, Dolnikowski GD, Bagley PJ, et al. A common mutation in the 5,10-methylenetetrahydrofolate reductase gene affects genomic DNA methylation through an interaction with folate status. Proc Natl Acad Sci. 2002;99:5606–5611
  10. Smith DEC, Kok RM, Teerlink T, Jakobs C, Smulders YM. Quantitative determination of the erythrocyte folate vitamer distribution by liquid chromatography–tandem mass spectrometry. Clin Chem Lab Med. 2006;44:450–459
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  14. Frosst P, Blom HJ, Milos R, Goyette P, Sheppard CA, Matthews RG, et al. A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase. Nat Genet. 1995;10:111–113
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  16. Bagley PJ, Selhub J. Analysis of folate form distribution by affinity followed by reversed-phase chromatography with electrochemical detection. Clin Chem. 2000;46:404–411
  17. Kutzbach C, Stokstad EL. Mammalian methylenetetrahydrofolate reductase. Partial purification, properties, and inhibition by S-adenosylmethionine. Biochim Biophys Acta. 1971;250:459–477
  18. McNulty H, McKinley MC, Wilson B, McPartlin J, Strain JJ, Weir DG, et al. Impaired functioning of thermolabile methylenetetrahydrofolate reductase is dependent on riboflavin status: implications for riboflavin requirements. Am J Clin Nutr. 2002;76:436–441
  19. van der Put NM, van den Heuvel LP, Steegers-Theunissen RP, Trijbels FJ, Eskes TK, Mariman EC, et al. Decreased methylene tetrahydrofolate reductase activity due to the 677C>T mutation in families with spina bifida offspring. J Mol Med. 1996;74:691–694
  20. Kim YI. Role of folate in colon cancer development and progression. J Nutr. 2003;133:3731s–3739s
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  23. Jacques PF, Kalmbach R, Bagley PJ, Russo GT, Rogers G, Wilson PWF, et al. The relationship between riboflavin and plasma total homocysteine in the Framingham offspring cohort is influenced by folate status and the C677T transition in the methylenetetrahydrofolate reductase gene. J Nutr. 2002;132:283–288
  24. Yamada K, Chen Z, Rozen R, Matthews RG. Effects of common polymorphisms on the properties of recombinant human methylenetetrahydrofolate reductase. Proc Natl Acad Sci U S A. 2001;98:14853–14858

☆ Y.M. Smulders is supported by a fellowship from the Netherlands Heart Foundation (grant 2001T044).

PII: S0955-2863(07)00014-9

doi: 10.1016/j.jnutbio.2006.11.010

The Journal of Nutritional Biochemistry
Volume 18, Issue 10 , Pages 693-699 , October 2007

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