The Journal of Nutritional Biochemistry
Volume 18, Issue 9 , Pages 623-628 , September 2007

l-Carnitine increases liver α-tocopherol and lowers liver and plasma triglycerides in aging ovariectomized rats

  • Richard M. Clark

      Affiliations

    • Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269-4017, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1 860 486 0121; fax: +1 860 486 3674.
  • ,
  • Anju Balakrishnan

      Affiliations

    • Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269-4017, USA
  • ,
  • David Waters

      Affiliations

    • Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269-4017, USA
  • ,
  • Dimple Aggarwal

      Affiliations

    • Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269-4017, USA
  • ,
  • Kevin Q. Owen

      Affiliations

    • Lonza, Inc., Allendale, NJ 07401, USA
  • ,
  • Sung I. Koo

      Affiliations

    • Department of Nutritional Sciences, University of Connecticut, Storrs, CT 06269-4017, USA

Received 28 August 2006 ,Revised 30 October 2006 ,Accepted 1 November 2006.

References 

  1. Hill K. The demography of menopause. Maturitas. 1996;23:113–127
  2. Kaplan JR, Manuck SB. Ovarian dysfunction, stress, and disease: a primate continuum. ILAR J. 2004;2:89–115
  3. Sowers MR, LaPietra MT. Menopause: its epidemiology and potential association with chronic diseases. Epidemiol Rev. 1995;17:287–302
  4. Ramsay RR. The carnitine acyltransferases: modulators of acyl-CoA-dependent reactions. Biochem Soc Trans. 2000;28:182–186
  5. Bremer J. Carnitine-metabolism and functions. Physiol Rev. 1983;63:1420–1480
  6. Rebouche CJ, Seim H. Carnitine metabolism and its regulation in microorganisms and mammals. Annu Rev Nutr. 1998;18:39–61
  7. Maccari F, Arseni A, Chiodi P, Ramacci MT, Angelucci L. Levels of carnitine in brain and other tissues of rats a of different ages: effect of acetyl-l-carnitine administration. Exp Gerontol. 1990;25:127–134
  8. Rebouche CJ. Carnitine function and requirements during the life cycle. FASEB J. 1992;6:3379–3386
  9. Costell M, O'Connor J, Grisolia S. Age-dependent decrease of carnitine content in muscle of mice and humans. Biochem Biophys Res Commun. 1989;161:1135–1143
  10. Karlic H, Lohninger S, Koeck T, Lohninger A. Dietary l-carnitine stimulates carnitine acyltransferases in the liver of aged rats. J Histochem Cytochem. 2002;50:205–212
  11. Kumaran S, Subathra M, Balu M, Panneerselvam C. Supplementation of l-carnitine improves mitochondrial enzymes in heart and skeletal muscle of aged rats. Exp Aging Res. 2005;31:55–67
  12. Zou W, Noh SK, Owen KQ, Koo SI. Dietary l-carnitine enhances the lymphatic absorption of fat and alpha-tocopherol in ovarietomized rats. J Nutr. 2005;135:753–756
  13. Voss AM, Reihart S, Sankarappa S, Sprecher H. The metabolism of 7,10,13,16,19-docosahexapentaenoic acid to 4,7,10,13,16,19-docosahexaenoic acid in rat liver is independent of a 4-desaturase. J Biol Chem. 1991;266:19995–20000
  14. Wanders RJA, Vreken P, Ferdinandusse S, Jansen GA, Waterham HR, vanRoermund CWT, et al. Peroxisomal fatty acid α- and β-oxidation in humans: enzymology, peroxisomal metabolite transporters and peroxisomal diseases. Biochem Soc Trans. 2001;29:250–267
  15. Washington L, Cook GA, Mansbach CM. Inhibition of carnitine palmitoyltransferase in the rat small intestine reduces export of triacylglycerol into the lymph. J Lipid Res. 2003;44:1395–1403
  16. Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993;23:1939–1951
  17. Rebouche CJ. Carnitine. In:  Shils ME,  Olson ME,  Shihe M,  Ross AC editor. Modern nutrition in health and disease. 9th ed.. Baltimore (Md): Lippincott Williams & Wilkins; 1999;p. 505–512
  18. Barua AB, Furr HC, Janick-Buckner D, Olson JA. Simultaneous analysis of individual carotenoids, retinol, retinyl esters, and tocopherols in serum by isocratic non aqueous reversed phase HPLC. Food Chem. 1993;46:419–424
  19. Folch J, Lees M, Sloane-Stanley GH. A simple method for the isolation and purification of total lipid from animal tissues. J Biol Chem. 1957;226:497–509
  20. Lepage G, Roy CC. Direct transesterification of all classes of lipids in one step reaction. J Lipid Res. 1986;27:114–120
  21. Allain C, Poon L, Chan C, Richmond W, Fu P. Enzymatic determination of total serum cholesterol. Clin Chem. 1974;20:470–475
  22. Carr T, Anderssen CJ, Rudel LL. Enzymatic determination of triglycerides, free cholesterol, and total cholesterol in tissue lipid extracts. Clin Biochem. 1993;26:39–42
  23. Clouet P, Sempore G, Tsoko M, Gresti J, Demarquoy J, Noit I, et al. Effect of short- and long-term treatments by a low level of dietary l-carnitine on parameters related to fatty acid oxidation in Wistar rat. Biochim Biophys Acta. 1996;1299:191–197
  24. Rabie MH, Szilagyi M, Gippert T, Votisky E, Gerendai D. Influence of dietary l-carnitine on performance and carcass quality of broiler chickens. Acta Biol Hung. 1997;48:241–252
  25. Melton SA, Keenan MJ, Stanciu CE, Hegsted M, Zablah-Pimentel EM, O'Neil CE, et al. l-Carnitine supplementation does not promote weight loss in ovariectomized rats despite endurance exercise. Int J Vitam Nutr Res. 2005;75:156–160
  26. Villani RG, Gannon J, Self M, Rich PA. l-Carnitine supplementation combined with aerobic training does not promote weight loss in moderately obese women. Int J Sport Nutr Exerc Metab. 2000;10:199–207
  27. Iossa S, Mollica MP, Lionetti L, Crescenzo R, Botta M, Barletta A, et al. Acetyl-l-carnitine supplementation differently influences nutrient partitioning, serum leptin concentration and skeletal muscle mitochondrial respiration in young and old rats. J Nutr. 2002;132:636–642
  28. Cullen P. Evidence that triglycerides are an independent coronary heart disease risk factor. Am J Cardiol. 2000;86:943–949
  29. Eskandari HG, Cimen MY, Tamer L, Kanik A, Atik U. Short term effects of l-carnitine on serum lipids in STZ-induced diabetic rats. Diabetes Res Clin Pract. 2004;66:129–132
  30. Terada R, Matsubara T, Koh N, Nakamura J, Hotta N. Effect of propionyl-l-carnitine on cardiac dysfunction in streptozotocin-diabetic rats. Eur J Pharmacol. 1998;357:185–191
  31. Tanaka Y, Sasaki R, Fukui F, Waki H, Kawabata T, Okazaki M, et al. Acetyl-l-carnitine supplementation restores decreased tissue carnitine levels and impaired lipid metabolism in aged rats. J Lipid Res. 2004;45:729–735
  32. Reddy JK, Rao MS. Liver metabolism and liver inflammation. II fatty liver disease and fatty acid oxidation. Am J Physiol Gastrointest Liver Physiol. 2006;290:852–858
  33. Dawson HD, Yamamoto Y, Zolfaghari R, Rosales FJ, Dietz J, Shimada T, et al. Regulation of hepatic vitamin A storage in a rat model of controlled vitamin A status during aging. J Nutr. 2000;130:1280–1286
  34. Sachan DS, Rhew TH, Bark RA. Amelioration effects of carnitine and its precursors on alcoholic-induced fatty liver. Am J Clin Nutr. 1984;39:738–744
  35. Bahcecioglu IH, Demir A, Ustundag B, Ilhan M, Baydas G, Canatan H, et al. Protective effect of l-carnitine on alcoholic fatty liver in rats. Med Sci Res. 1999;27:475–478
  36. Dayanandan A, Kumar P, Panneerselvam C. Protective role of l-carnitine on liver and heart lipid peroxidation in atherosclerotic rats. J Nutr Biochem. 2001;12:254–257
  37. Kalaiselvi T, Panneerselvam C. Effect of l-carnitine on the status of lipid peroxidation and antioxidants in aging rats. J Nutr Biochem. 1998;9:575–581
  38. Calabrese V, Stella AMG, Calvani M, Butterfield DA. Acetycarnitine and cellular stress response: roles in nutritional redox homeostasis and regulation of longevity genes. J Nutr Biochem. 2006;17:73–88
  39. Doh KO, Kim YW, Park SY, Lee SK, Park JS, Kim JY. Interrelation between long-chain fatty acid oxidation rate and carnitine palmitoyltransferase 1 activity with different isoforms in rat tissues. Life Sci. 2005;77:435–443
  40. Green MH, Green JB, Lewis KC. Variation in retinol utilization rate with vitamin A status in the rat. J Nutr. 1987;117:694–703
  41. Verma R, Beaton G. Quantitative aspects of urinary and fecal excretion of radioactive metabolites of vitamin A in the rat. Can J Physiol Pharmacol. 1972;50:1026–1037
  42. Hicks VA, Gunning DB, Olson JA. Metabolism, plasma transport and biliary excretion of radioactive vitamin A and its metabolites as a function of liver reserves of vitamin A in the rat. J Nutr. 1984;114:1327–1333

PII: S0955-2863(07)00006-X

doi: 10.1016/j.jnutbio.2006.11.007

The Journal of Nutritional Biochemistry
Volume 18, Issue 9 , Pages 623-628 , September 2007