The Journal of Nutritional Biochemistry
Volume 18, Issue 8 , Pages 533-540 , August 2007

l-Carnitine attenuates oxidative stress in hypertensive rats

  • Lucía Gómez-Amores

      Affiliations

    • Department of Physiology and Zoology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • These authors contributed equally to this work.
    • ,
  • Alfonso Mate

      Affiliations

    • Department of Physiology and Zoology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • These authors contributed equally to this work.
    • ,
  • José L. Miguel-Carrasco

      Affiliations

    • Department of Physiology and Zoology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • ,
  • Luís Jiménez

      Affiliations

    • Department of Clinical Biochemistry, Virgen del Rocío Hospital, E-41013 Seville, Spain
    • ,
  • Ángeles Jos

      Affiliations

    • Department of Biochemistry and Toxicology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • ,
  • Ana M. Cameán

      Affiliations

    • Department of Biochemistry and Toxicology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • ,
  • Elisa Revilla

      Affiliations

    • Department of Biochemistry and Toxicology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • ,
  • Consuelo Santa-María

      Affiliations

    • Department of Biochemistry and Toxicology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • ,
  • Carmen M. Vázquez

      Affiliations

    • Department of Physiology and Zoology, Faculty of Pharmacy, University of Seville, E-41012 Seville, Spain
    • Corresponding Author InformationCorresponding author. Dpto. Fisiología y Zoología, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González, 2, E-41012 Sevilla, Spain. Tel.: +34 954 556 775; fax: +34 954 233 765.

Received 8 June 2006 ,Revised 8 September 2006 ,Accepted 2 October 2006.

References 

  1. Lassegue B, Griendling KK. Reactive oxygen species in hypertension; an update. Am J Hypertens. 2004;17:852–860
  2. Drexler H. Nitric oxide and coronary endothelial dysfunction in humans. Cardiovasc Res. 1999;43:572–579
  3. Lembo G, Vecchione C, Izzo R, Fratta L, Fontana D, Marino G, et al. Noradrenergic vascular hyper-responsiveness in human hypertension is dependent on oxygen free radical impairment of nitric oxide activity. Circulation. 2000;102:552–557
  4. Wu R, Millette E, Wu L, de Champlain J. Enhanced superoxide anion formation in vascular tissues from spontaneously hypertensive and desoxycorticosterone acetate–salt hypertensive rats. J Hypertens. 2001;19:741–748
  5. Ulker S, McMaster D, McKeown PP, Bayraktutan U. Impaired activities of antioxidant enzymes elicit endothelial dysfunction in spontaneous hypertensive rats despite enhanced vascular nitric oxide generation. Cardiovasc Res. 2003;59:488–500
  6. Munzel T, Hink U, Heitzer T, Meinertz T. Role for NADPH/NADH oxidase in the modulation of vascular tone. Ann N Y Acad Sci. 1999;874:386–400
  7. Zalba G, San Jose G, Moreno MU, Fortuno MA, Fortuno A, Beaumont FJ, et al. Oxidative stress in arterial hypertension: role of NAD(P)H oxidase. Hypertension. 2001;38:1395–1399
  8. Somova LI, Nadar A, Gregory M, Khan N. Antioxidant status of the hypertrophic heart of Dahl hypertensive rat as a model for evaluation of antioxidants. Methods Find Exp Clin Pharmacol. 2001;23:5–12
  9. Cediel E, Sanz-Rosa D, Oubina MP, de las HN, Gonzalez Pacheco FR, Vegazo O, et al. Effect of AT1 receptor blockade on hepatic redox status in SHR: possible relevance for endothelial function?. Am J Physiol Regul Integr Comp Physiol. 2003;285:R674–R681
  10. Midaoui AE, Elimadi A, Wu L, Haddad PS, de Champlain J. Lipoic acid prevents hypertension, hyperglycemia, and the increase in heart mitochondrial superoxide production. Am J Hypertens. 2003;16:173–179
  11. Schnackenberg CG, Welch WJ, Wilcox CS. Normalization of blood pressure and renal vascular resistance in SHR with a membrane-permeable superoxide dismutase mimetic: role of nitric oxide. Hypertension. 1998;32:59–64
  12. Schnackenberg CG, Wilcox CS. Two-week administration of tempol attenuates both hypertension and renal excretion of 8-Iso prostaglandin f2alpha. Hypertension. 1999;33:424–428
  13. Chen X, Touyz RM, Park JB, Schiffrin EL. Antioxidant effects of vitamins C and E are associated with altered activation of vascular NADPH oxidase and superoxide dismutase in stroke-prone SHR. Hypertension. 2001;38:606–611
  14. Kalaiselvi T, Panneerselvam C. Effect of l-carnitine on the status of lipid peroxidation and antioxidants in aging rats. J Nutr Biochem. 1998;9:575–581
  15. Arockia P, Rani J, Panneerselvam C. Carnitine as a free radical scavenger in aging. Exp Gerontol. 2001;36:1713–1726
  16. Sushamakumari S, Jayadeep A, Kumar JS, Menon VP. Effect of carnitine on malondialdehyde, taurine and glutathione levels in heart of rats subjected to myocardial stress by isoproterenol. Indian J Exp Biol. 1989;27:134–137
  17. Hariri RJ, Alonso DR, Hajjar DP, Coletti D, Weksler ME. Aging and arteriosclerosis: I. Development of myointimal hyperplasia after endothelial injury. J Exp Med. 1986;164:1171–1178
  18. Dayanandan A, Kumar P, Panneerselvam C. Protective role of l-carnitine on liver and heart lipid peroxidation in atherosclerotic rats. J Nutr Biochem. 2001;12:254–257
  19. Sayed-Ahmed MM, Khattab MM, Gad MZ, Mostafa N. l-Carnitine prevents the progression of atherosclerotic lesions in hypercholesterolaemic rabbits. Pharmacol Res. 2001;44:235–242
  20. Bueno R, Alvarez dS, Perez-Guerrero C, Gomez-Amores L, Vazquez CM, Herrera MD. l-Carnitine and propionyl-l-carnitine improve endothelial dysfunction in spontaneously hypertensive rats: different participation of NO and COX-products. Life Sci. 2005;77:2082–2097
  21. Rauchova H, Dobesova Z, Drahota Z, Zicha J, Kunes J. The effect of chronic l-carnitine treatment on blood pressure and plasma lipids in spontaneously hypertensive rats. Eur J Pharmacol. 1998;342:235–239
  22. Singh RB, Niaz MA, Agarwal P, Beegum R, Rastogi SS, Sachan DS. A randomised, double-blind, placebo-controlled trial of l-carnitine in suspected acute myocardial infarction. Postgrad Med J. 1996;72:45–50
  23. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951;193:265–275
  24. Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun. 1976;71:952–958
  25. Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Biol Chem. 1975;250:5475–5480
  26. McCord JM, Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein). J Biol Chem. 1969;244:6049–6055
  27. Beers RF, Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem. 1952;195:133–140
  28. Esterbauer H, Cheeseman KH. Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol. 1990;186:407–421
  29. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci (Lond). 1993;84:407–412
  30. Marzinzig M, Nussler AK, Stadler J, Marzinzig E, Barthlen W, Nussler NC, et al. Improved methods to measure end products of nitric oxide in biological fluids: nitrite, nitrate, and s-nitrosothiols. Nitric Oxide. 1997;1:177–189
  31. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–685
  32. Mate A, Barfull A, Hermosa AM, Gomez-Amores L, Vazquez CM, Planas JM. Regulation of sodium-glucose cotransporter SGLT1 in the intestine of hypertensive rats. Am J Physiol Regul Integr Comp Physiol. 2006;291:R760–R767
  33. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Anal Biochem. 1987;162:156–159
  34. Suzuki H, Swei A, Zweifach BW, Schmid-Schonbein GW. In vivo evidence for microvascular oxidative stress in spontaneously hypertensive rats. Hydroethidine microfluorography. Hypertension. 1995;25:1083–1089
  35. Tsikas D. Methods of quantitative analysis of the nitric oxide metabolites nitrite and nitrate in human biological fluids. Free Radic Res. 2005;39:797–815
  36. Ito H, Torii M, Suzuki T. A comparative study on defense systems for lipid peroxidation by free radicals in spontaneously hypertensive and normotensive rat myocardium. Comp Biochem Physiol B. 1992;103:37–40
  37. Kitts DD, Yuan YV, Godin DV. Plasma and lipoprotein lipid composition and hepatic antioxidant status in spontaneously hypertensive (SHR) and normotensive (WKY) rats. Can J Physiol Pharmacol. 1998;76:202–209
  38. Newaz MA, Nawal NN. Effect of alpha-tocopherol on lipid peroxidation and total antioxidant status in spontaneously hypertensive rats. Am J Hypertens. 1998;11:1480–1485
  39. Vaziri ND, Ni Z, Oveisi F, Trnavsky-Hobbs DL. Effect of antioxidant therapy on blood pressure and NO synthase expression in hypertensive rats. Hypertension. 2000;36:957–964
  40. Digiesi V, Lenuzza M, Digiesi G. Prospects for the use of antioxidant therapy in hypertension. Ann Ital Med Int. 2001;16:93–100
  41. Gomez-Amores L, Mate A, Revilla E, Santa-Maria C, Vazquez CM. Antioxidant activity of propionyl-l-carnitine in liver and heart of spontaneously hypertensive rats. Life Sci. 2006;78:1945–1952
  42. Dayal S, Brown KL, Weydert CJ, Oberley LW, Arning E, Bottiglieri T, et al. Deficiency of glutathione peroxidase-1 sensitizes hyperhomocysteinemic mice to endothelial dysfunction. Arterioscler Thromb Vasc Biol. 2002;22:1996–2002
  43. Digiesi V, Cantini F, Bisi G, Guarino G, Brodbeck B. l-Carnitine adjuvant therapy in essential hypertension. Clin Ter. 1994;144:391–395
  44. Fukui T, Ishizaka N, Rajagopalan S, Laursen JB, Capers Q, Taylor WR, et al. Griendling, p22phox mRNA expression and NADPH oxidase activity are increased in aortas from hypertensive rats. Circ Res. 1997;80:45–51
  45. Ushio-Fukai M, Zafari AM, Fukui T, Ishizaka N, Griendling KK. p22phox is a critical component of the superoxide-generating NADH/NADPH oxidase system and regulates angiotensin II-induced hypertrophy in vascular smooth muscle cells. J Biol Chem. 1996;271:23317–23321
  46. Virdis A, Neves MF, Amiri F, Viel E, Touyz RM, Schiffrin EL. Spironolactone improves angiotensin-induced vascular changes and oxidative stress. Hypertension. 2002;40:504–510

PII: S0955-2863(06)00233-6

doi: 10.1016/j.jnutbio.2006.10.004

The Journal of Nutritional Biochemistry
Volume 18, Issue 8 , Pages 533-540 , August 2007

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