The Journal of Nutritional Biochemistry
Volume 18, Issue 5 , Pages 341-347 , May 2007

Epigallocatechin-3-gallate increases the formation of mineralized bone nodules by human osteoblast-like cells

  • Bahareh Vali

      Affiliations

    • Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 3E2
  • ,
  • Leticia G. Rao

      Affiliations

    • Calcium Research Laboratory, Division of Endocrinology and Metabolism, St. Michael's Hospital and Department of Medicine, University of Toronto, Toronto, Ontario, Canada M5B 1A6
  • ,
  • Ahmed El-Sohemy

      Affiliations

    • Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada M5S 3E2
    • Corresponding Author InformationCorresponding author. Tel.: +1 416 946 5776; fax: +1 416 978 5882.

Received 11 March 2006 ,Revised 26 June 2006 ,Accepted 27 June 2006.

References 

  1. Hegarty VM, Helen MM, Khaw K. Tea drinking and bone mineral density in older women. Am J Clin Nutr. 2000;71:1003–1007
  2. Hoover PA, Webber CE, Beaumont LF, Blake JM. Postmenopausal bone mineral density: relationship to calcium intake, calcium absorption, residual estrogen, body composition and physical activity. Can J Physiol Pharmacol. 1996;74:911–917
  3. Kanis J, Johnell O, Gullberg B, Allande E, Elffors L, Ranstam J, et al. Risk factors for hip fracture in men from southern Europe: the MEDOS Study. Mediterranean Osteoporosis Study. Osteoporos Int. 1999;9:45–54
  4. Johnell O, Gullberg B, Kanis JA, Allander E, Elffors L, Dequeker J, et al. Risk factors for hip fracture in European women: the MEDOS Study. Mediterranean Osteoporosis Study. J Bone Miner Res. 1995;10:1802–1815
  5. Chen Z, Pettinger MB, Ritenbaugh C, LaCronix AZ, Robbins J, Caan BJ, et al. Habitual tea consumption and risk of osteoporosis: a prospective study in the women's health initiative observational cohort. Am J Epidemiol. 2003;158:772–781
  6. Wu CH, Yang YC. Epidemiological evidence of increased bone mineral density in habitual tea drinkers. Arch Intern Med. 2002;162:1001–1006
  7. Yang CS, Landau JM. Effects of tea consumption on nutrition and health. J Nutr. 2000;130:2409–2412
  8. Brown MD. Green tea (Camellia sinensis) extracts and its possible role in the prevention of cancer. Altern Med Rev. 1999;4:360–370
  9. Sato T, Miyata G. The nutraceutical benefit: part I. Green tea. Nutrition. 2000;16:315–317
  10. Higdon JV, Frei B. Tea catechins and polyphenols: health effects, metabolism and antioxidant function. Crit Rev Food Sci Nutr. 2003;43:89–143
  11. Riggs BL, Melton LJ. The prevention and treatment of osteoporosis. N Engl J Med. 1992;327:620–627
  12. Aubin JE. Advances in osteoblast lineage. Biochem Cell Biol. 1998;76:899–910
  13. Lian BJ, Stein GS, Canalis E, Robey PG, Boskey AL. Bone formation: osteoblast lineage cells, growth factors, matrix proteins and the mineralization process. In:  Favus MJ editors. Primer on the metabolic bone disease and disorder of mineral metabolism. Philadelphia: Lippincott Williams & Williams; 1999;
  14. Ducy P, Zhang R, Geoffroy V. Osf2/Cbfa1: a transcriptional activator of osteoblast differentiation. Cell. 1997;89:747–754
  15. Komori T, Yagi H, Nomura S. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell. 1997;89:755–764
  16. Otto F, Thornell AP, Crompton T. Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell. 1997;89:765–771
  17. Liu W, Toyosawa S, Furuichi T, Kanatani N, Yoshida C, Liu Y, et al. Overexpression of Cbfa1 in osteoblasts inhibits osteoblast maturation and causes osteopenia with multiple fractures. J Cell Biol. 2001;155:157–166
  18. Harada H, Tagashira S, Fujiwara M, Ogawa S, Katsumata T, Yamaguchi A, et al. Cbfa1 isoforms exert functional differences in osteoblast differentiation. J Biol Chem. 1999;274:6972–6978
  19. Sudhakar S, Katz MS, Elango N. Analysis of type-I and type-II RUNX2 protein expression in osteoblasts. Biochem Biophys Res Commun. 2001;286:74–79
  20. Rodan SB, Imai Y, Thiede MA, Wesolowski G, Thomson D, Bar-Shavit Z, et al. Characterization of a human osteosarcoma cell line SaOS-2 with osteoblastic properties. Cancer Res. 1987;47:49–66
  21. Rao LG, Murray TM. Studies of human osteoblasts in vitro: estrogen actions and interactions with other hormones at different stages of differentiation. Drug Dev Res. 2000;49:174–186
  22. Nakagawa H, Wachi M, Woo J, Kato M, Kasai S, Takahashi F, et al. Fenton reaction is primarily involved in a mechanism of (−)-epigallocatechin-3-gallate to induce osteoclastic cell death. Biochem Biophys Res Commun. 2002;292:94–101
  23. Yun JH, Pang EK, Kim CS, Yoo YJ, Cho KS, Chai JK, et al. Inhibitory effects of green tea polyphenol (−)-epigallocatechin gallate on the expression of matrix metalloproteinase-9 and on the formation of osteoclasts. J Periodontal Res. 2004;39(5):300–307
  24. Rao LG, Liu LJ, Murray TM, McDermott E, Zhang X. Estrogen added intermittently but not continuously, stimulates differentiation and bone formation in SaOS-2 cells. Biol Pharm Bull. 2003;26:936–945
  25. Rao LG, Liu LJ, Murray TM, McDermott E. 17-B estradiol stimulates mineralized bone formation when added intermittently to SaOS-2 cells. Drug Metabol Drug Interact. 2001;18:149–158
  26. Hale LV, Ma YF, Santerre RF. Semi-quantitative fluorescence analysis of calcein binding as a measurement of in vitro mineralization. Calcif Tissue Int. 2000;67:80–84
  27. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem. 1976;72:248–254
  28. Choi JH, Rhee IK, Park KY, Kim JK, Rhee SJ. Action of green tea catechin on bone metabolism disorder in chronic cadmium-poisoned rats. Life Sci. 2003;73:1479–1489
  29. Das AS, Mukherjee M, Mitra C. Evidence for a prospective anti-osteoporosis effect of black tea (Camellia sinensis) extract in a bilaterally ovariectomized rat model. Asia Pac J Clin Nutr. 2004;13:210–216
  30. Kung-Sutherland MS, Rao LG, Muzzafar SA, Wylie JN, Wong MM, McBroom R, et al. Age-dependent expression of osteoblastic phenotypic markers in normal human osteoblasts cultured long term in the presence of dexamethasone. Osteoporos Int. 1995;5:335–343
  31. Kassem M, Risteli L, Mosekilde L, Melsen F, Eriksen EF. Formation of osteoblast-like cells from human mononuclear bone marrow cultures. APMIS. 1991;99:269–274
  32. Robey PM, Termine JD. Human bone cells in vitro. Calcif Tissue Int. 1985;37:453–460
  33. Wong MM, Rao LG, Ly H, Hamilton L, Sturtridge W, McBroom R, et al. Long term effects of physiological concentrations of dexamethasone on human bone-derived cells. J Bone Miner Res. 1990;5:803–813
  34. Matsuyama T, Lau K, Wergedal JE. Monolayer cultures of normal human bone cells contain multiple subpopulations of alkaline phosphatase positive cells. Calcif Tissue Int. 1990;47:276–283
  35. Manduca P, Sanguineti C, Pistone M, Boccignone E, Sanguineti F, Santolini F, et al. Differential expression of alkaline phosphatase in clones of human osteoblast-like cells. J Bone Miner Res. 1993;8:291–300
  36. Rao LG, Wylie JN, Kung-Sutherland MS, Murray TM. 17 Beta estradiol enhances the stimulatory effect of 1,25 dihydroxy vitamin D3 on alkaline phosphatase activity in human osteosarcoma SaOs-2 cells in a differentiation-dependent manner. J Endocrinol. 1996;148:181–187
  37. Rao LG, Wylie JN, Kung-Sutherland MS, Murray TM. 17 Beta-estradiol and parathyroid hormone potentiate each others stimulatory effects on alkaline phosphatase activity in SaOS-2 cells in a differentiation-dependent manner. Endocrinology. 1994;134:614–620
  38. Meloan SN, Puchtler H. Chemical mechanisms of staining methods: von Kossa's technique. What von Kossa really wrote and a modified reaction for selective demonstration of inorganic phosphate. J Histotechnol. 1985;8:11–13
  39. Aubin JE. Regulation of osteoblast formation and function. Rev Endocr Metab Disord. 2001;2:81–94
  40. Maeda T, Matsunuma A, Kawane T, Horiuchi N. Simvastatin promotes osteoblast differentiation and mineralization in MC3T3-E1 cells. Biochem Biophys Res Commun. 2000;280:874–877
  41. Banarjee C, McCabe LR, Choi JY, Hiebert SW, Stein JL, Stein GS, et al. Runt homology domain proteins in osteoblasts differentiation: AML3/Cbfa1 is a major component of bone-specific complex. J Cell Biochem. 1997;66:1–8
  42. Kobayashi H, Gao Y, Ueta C, Yamaguchi A, Komori T. Multilineage differentiation of Cbfa1-deficient calvarial cells in vitro. Biochem Biophys Res Commun. 2000;273:630–636

PII: S0955-2863(06)00163-X

doi: 10.1016/j.jnutbio.2006.06.005

The Journal of Nutritional Biochemistry
Volume 18, Issue 5 , Pages 341-347 , May 2007