Effects of biotin on growth and protein biotinylation in Saccharomyces cerevisiae✩
Received 30 March 2005; received in revised form 30 March 2005; accepted 30 March 2005.
Abstract
In mammals, biotin, well known for its role as the cofactor of carboxylases, also controls the expression not only of proteins involved in this function, but also of a large number and variety of other different proteins. As a first step towards looking for a rationale for these phenomena, we intend to compare these regulatory functions of biotin between the rat and the much less evolutionized eukaryote, Saccharomyces cerevisiae. Thus far, we have measured growth in yeast cultured on different concentrations of biotin to choose the experimental conditions to be used (2, 200 and 2000 μM) and have found that a band corresponding to the biotinylated S. cerevisiae Arc1p protein appears at streptavidin Western blots at a biotin concentration above 2000 μM, its density increasing with higher biotin amounts. We will now study changes in yeast transcriptome with these varying concentrations and compare them with changes observed in the rat.
aUnidad de Genética de la Nutrición, Instituto de Investigaciones Biomédicas, UNAM, Mexico, D.F. 04530, Mexico
bInstituto Nacional de Pediatría, Av. del IMAN No. 1, 4o. piso, Mexico, D.F. 04530, Mexico
cInstituto de Fisiología Celular, UNAM, Apartado Postal 70-600, Mexico, D.F. 04510, Mexico
Corresponding author. Torre de Investigación, INP, Av. del IMAN No. 1, 4o. piso, Mexico, D.F. 04530, Mexico. Tel.: +52 55 5606 3489; fax: +52 55 5606 3489.
✩ This paper was presented at the “International Symposium: Vitamins as Regulators of Genetic Expression: Biotin as a Model” NAFTA Satellite Meeting to the XXV National Congress of Biochemistry held December 3–4, 2004, in Ixtapa, Zihuatanejo, Mexico. This meeting was sponsored by Sociedad Mexicana de Bioquimica A.C.; Programa de Doctorado en Ciencias Biomedicas, Universidad Nacional Autonoma de Mexico; Laboratorios Roche-Syntex, Mexico; and Instituto de Investigaciones Biomedicas, Universidad Nacional Autonoma de Mexico.
ABSTRACT