The Journal of Nutritional Biochemistry
Volume 12, Issue 11 , Pages 622-630 , November 2001

The role of Δ9-desaturase in the production of cis-9, trans-11 CLA

Presented at the annual meeting of the American Dairy Science Association in 2000; Corl, B.A., Baumgard, L.H., Dwyer, D.A., Griinari, J.M., Phillips, B.S., and Bauman, D.E., The role of Δ9-desaturase in the production of cis-9, trans-11 CLA and other Δ9-desaturated fatty acids in milk fat. J. Dairy Sci. 83(suppl. 1):164. Baltimore, MD.

  • Benjamin A. Corl

      Affiliations

    • Department of Animal Science, Cornell University, 262 Morrison Hall, Ithaca, NY 14853-4801, USA
    • ,
  • Lance H. Baumgard

      Affiliations

    • Department of Animal Science, Cornell University, 262 Morrison Hall, Ithaca, NY 14853-4801, USA
    • ,
  • Debra A. Dwyer

      Affiliations

    • Department of Animal Science, Cornell University, 262 Morrison Hall, Ithaca, NY 14853-4801, USA
    • ,
  • J.Mikko Griinari

      Affiliations

    • Department of Animal Science, University of Helsinki, Helsinki, Finland
    • ,
  • Bliss S. Phillips

      Affiliations

    • National Center for Agricultural Utilization Research, ARS/USDA, Peoria, IL, USA
    • ,
  • Dale E. Bauman

      Affiliations

    • Department of Animal Science, Cornell University, 262 Morrison Hall, Ithaca, NY 14853-4801, USA
    • Corresponding Author InformationCorresponding author. Tel.:+(607) 255-2262; fax: +(607) 255-9829

Received 28 February 2001 ,Revised 5 June 2001 ,Accepted 3 July 2001.

References 

  1. National Research Council. Opportunities in the Nutrition and Food Sciences. Washington, DC: National Academy Press; 1994;
  2. M.A. McGuire, M.K. McGuire, Conjugated linoleic acid (CLA): A ruminant fatty acid with beneficial effects on human health. Proc. Am. Soc. Anim. Sci. (2000) http://www.asas.org/jas/symposia/proceeedings/0938.pdf.
  3. Ip C, Banni S, Angioni E, Carta G, McGinley J, Thompson HJ, et al. Conjugated linoleic acid-enriched butter alters mammary gland morphogenesis and reduces cancer risk in rats. J. Nutr. 1999;129:2135–2142
  4. Griinari JM, Bauman DE. Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In:  Yurawecz M,  Mossoba MM,  Kramer JKG,  Pariza MW,  Nelson GJ editor. Advances in Conjugated Linoleic Acid Research, Vol. 1. Champaign, IL: AOCS Press; 1999;p. 180–200
  5. Griinari JM, Corl BA, Lacy SH, Chouinard PY, Nurmela KVV, Bauman DE. Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Δ9-desaturase. J. Nutr. 2000;130:2285–2291
  6. Santora JE, Palmquist DL, Roehrig KL. Trans-Vaccenic acid is desaturated to conjugated linoleic acid in mice. J. Nutr. 2000;130:208–215
  7. Salminen I, Mutanen M, Jauhiainen M, Aro A. Dietary trans fatty acids increase conjugated linoleic acid levels in human serum. J. Nutr. Biochem. 1998;9:93–98
  8. Adlof RO, Duval S, Emken EA. Biosynthesis of conjugated linoleic acid in humans. Lipids. 2000;35:131–135
  9. Jeffcoat R, Pollard MR. Studies on the inhibition of the desaturases by cyclopropene fatty acids. Lipids. 1977;12:480–485
  10. Fox DG, Sniffen CJ, O’Connor JD, Russell JB, Van Soest PJ. A net carbohydrate and protein system for evaluating cattle diets (III. Cattle requirements and diet adequacy). J. Anim. Sci. 1992;70:3578–3596
  11. National Research Council. Nutrient requirements of dairy cattle. 6th rev. ed.. Washington, DC: National Academy of Sciences; 1989;
  12. Association of Official Analytical Chemists. Official Methods of Analysis of AOAC International. 16th ed., 4th rev.. Gaithersburg, MD: AOAC International; 1998;
  13. Chouinard PY, Corneau L, Saebo A, Bauman DE. Milk yield and composition during abomasal infusion of conjugated linoleic acid. J. Dairy Sci. 1999;82:2737–2745
  14. Spires HR, Clark JH, Derrig RG, Davis CL. Milk production and nitrogen utilization in response to postruminal infusion of sodium caseinate in lactating cows. J. Nutr. 1975;105:1111–1115
  15. Hara A, Radin NS. Lipid extraction of tissues with a low-toxicity solvent. Anal. Biochem. 1978;90:420–426
  16. Christie WW. A simple procedure for rapid transmethylation of glycerolipids and cholesteryl esters. J. Lipid Res. 1982;23:1072–1075
  17. Hashimoto N, Aoyama T, Shiori T. New methods and reagents in organic synthesis. 14. A simple efficient preparation of methyl esters with trimethylsilyldiazomethane (TMSCHN2) and its application to gas chromatographic analysis of fatty acids. Chem. Pharm. Bull. 1981;29:1475–1478
  18. SAS Institute Inc.. SAS/STAT User’s Guide, Version 6. 4th rev. ed.. Cary, N.C: SAS Institute; 1989;
  19. Bauman DE, Corl BA, Baumgard LH, Griinari JM. Conjugated linoleic acid (CLA) and the dairy cow. In:  Garnsworthy PC,  Cole DJ editor. Recent Advances in Animal Nutrition. Nottingham, UK: Nottingham University Press; 2001;p. 237–266
  20. Parodi PW. Cow’s milk fat components as potential anticarcinogenic agents. J. Nutr. 1997;127:1055–1060
  21. Bauman DE, Davis CL. Biosynthesis of milk fat. In:  Larson BL,  Smith VR editor. Lactation (A Comprehensive Treatise). New York, NY: Academic Press; 1974;p. 31–75
  22. Phelps RA, Shenstone FS, Kemmerer AR, Evans RJ. A review of cyclopropene compounds (biological effects of some derivatives). Poult. Sci. 1965;44:358–394
  23. Cook LJ, Scott TW, Mills SC, Fogerty AC, Johnson AR. Effects of protected cyclopropene fatty acids on the composition of ruminant milk fat. Lipids. 1976;11:705–711
  24. Bickerstaffe R, Johnson AR. The effect of intravenous infusions of sterculic acid on milk fat synthesis. Br. J. Nutr. 1972;27:561–570
  25. P.A. Porter, An investigation of the possible role of trans-fatty acids in the low milk fat syndrome (LFMS) of the dairy cow, Master’s thesis, Cornell University, Ithaca, NY, 1984.
  26. Ward RJ, Travers MT, Richards SE, Vernon RG, Salter AM, Buttery PJ, et al. Stearoyl-CoA desaturase mRNA is transcribed from a single gene in the ovine genome. Biochem. Biophys. Acta. 1998;1391:145–156
  27. Kinsella JE. Stearyl CoA as a precursor of oleic acid and glycerolipids in mammary microsomes from lactating bovine (possible regulatory step in milk triglyceride synthesis). Lipids. 1972;7:349–355
  28. McDonald TM, Kinsella JE. Stearyl-CoA desaturase of bovine mammary microsomes. Arch. Biochem. Biophys. 1973;156:223–231
  29. Bickerstaffe R, Annison EF. The desaturase activity of goat and sow mammary tissue. Comp. Biochem. Physiol. 1970;35:653–665
  30. Bickerstaffe R, Annison EF. Glycerokinase and desaturase activity in pig, chicken and sheep intestinal epithelium. Comp. Biochem. Physiol. 1969;31:47–54
  31. St. John LC, Lunt DK, Smith SB. Fatty acid elongation and desaturation enzyme activities of bovine liver and subcutaneous adipose tissue microsomes. J. Anim. Sci. 1991;69:1064–1073
  32. Ntambi JM. The regulation of stearoyl-CoA desaturase (SCD). Prog. Lip. Res. 1995;34:139–150
  33. Chung M, Ha S, Jeong S, Bok J, Cho K, Baik M, et al. Cloning and characterization of bovine stearoyl CoA desaturase1 cDNA from adipose tissues. Biosci. Biotechnol. Biochem. 2000;64:1526–1530
  34. Yurawecz MP, Roach JAG, Sehat N, Mossoba MM, Kramer JKG, Fritsche J, et al. A new conjugated linoleic acid isomer, 7 trans, 9 cis-octadecadienoic acid, in cow milk, cheese, beef and human milk and adipose tissue. Lipids. 1998;33:803–809
  35. Chilliard Y, Ferlay A, Mansbridge RM, Doreau M. Ruminant milk fat plasticity (nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids). Ann. Zootech. 2000;49:181–235

PII: S0955-2863(01)00180-2

The Journal of Nutritional Biochemistry
Volume 12, Issue 11 , Pages 622-630 , November 2001

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