The Journal of Nutritional Biochemistry
Volume 12, Issue 11 , Pages 610-621 , November 2001

Acyl-CoA: retinol acyltransferase (ARAT) and lecithin:retinol acyltransferase (LRAT) activation during the lipocyte phenotype induction in hepatic stellate cells

  • Vitor A Fortuna

      Affiliations

    • Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Rio de Janeiro, Brazil
    • Departamento de Bioquı́mica, Instituto de Quı́mica, Rio de Janeiro, Brazil
  • ,
  • Luiz C Trugo

      Affiliations

    • Departamento de Bioquı́mica, Instituto de Quı́mica, Rio de Janeiro, Brazil
  • ,
  • Radovan Borojevic

      Affiliations

    • Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Rio de Janeiro, Brazil
    • Programa Avançado de Biologia Celular Aplicada à Medicina, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
    • Corresponding Author InformationCorresponding author. Tel.: +5521-2562-6484; fax: +5521-2562-6483

Received 4 January 2001 ,Revised 4 June 2001 ,Accepted 2 July 2001.

References 

  1. Ross AC. Cellular metabolism and activation of retinoids (roles of cellular retinol-binding proteins). FASEB J. 1993;7:317–327
  2. Blaner WS, Olson JA. Retinol and retinoic acid metabolism. In:  Sporn MB,  Roberts AB,  Goodman DS editor. The Retinoids (Biology, Chemistry, and Medicine). 2nd edn.. New York, NY, USA: Raven Press; 1994;p. 229–255
  3. Blaner WS, Hendriks HFJ, Brouwer A, de Leeuw AM, Knook DL, Goodman DS. Retinoids, retinoid-binding proteins, and retinyl palmitate hydrolase distributions in different types of rat liver cells. J. Lipid Res. 1985;26:1241–1251
  4. Harrison EH, Blaner WS, Goodman DS, Ross AC. Subcellular localization of retinoids, retinoid-binding proteins, and acyl-CoA:retinol acyltransferase in rat liver. J. Lipid Res. 1987;28:973–981
  5. Senoo H, Imai K, Matano Y, Sato M. Molecular mechanisms in the reversible regulation of morphology, proliferation and collagen metabolism in hepatic stellate cells by the three-dimensional structure of the extracellular matrix. J. Gastro. Hepatol. 1998;13:S19–S32
  6. Blomhoff R, Rasmussen M, Nilsson A, Norum KR, Berg T, Blaner WS, et al. Hepatic retinol metabolism. J. Biol. Chem. 1985;260:13560–13565
  7. Noy N, Blaner WS. Interactions of retinol with binding proteins (studies with rat cellular retinol-binding protein and with rat retinol-binding protein). Biochemistry. 1991;30:6380–6386
  8. Ong DE, Newcomer ME, Chytil F. Cellular retinoid-binding proteins. In:  Sporn MB,  Roberts AB,  Goodman DS editor. The Retinoids (Biology, Chemistry, and Medicine). 2nd edn.. New York, NY, USA: Raven Press; 1994;p. 303–304
  9. Napoli JL. Interactions of retinoid binding proteins and enzymes in retinoid metabolism. Biochim. Biophys. Acta. 1999;1440:139–162
  10. Matsuura T, Nagamori S, Hasumura S, Sujino H, Niiya M, Shimizu K. Regulation of vitamin A transport into cultured stellate cells of rat liver (studies by anchored cell analysis and sorting system). Exp. Cell. Res. 1993;209:33–37
  11. Matsuura T, Hasumura S, Nagamori S, Murakami K. Retinol esterification activity contributes to retinol transport in stellate cells. Cell. Struct. Funct. 1999;24:111–116
  12. Herr FM, Ong DE. Differential interaction of lecithin (retinol acyltransferase with cellular retinoid-binding proteins). Biochemistry. 1992;31:6748–6755
  13. Ruiz A, Winston A, Lim YH, Gilbert BA, Rando RR, Bok D. Molecular and biochemical characterization of lecithin retinol acyltransferase (LRAT). J. Biol. Chem. 1999;274:3834–3841
  14. Randolph RK, Winkler KE, Ross AC. Fatty acyl CoA-dependent and -independent retinol esterification by rat liver and lactating mammary gland microsomes. Arch. Biochem. Biophys. 1991;288:500–508
  15. Dawson HD, Yamamoto Y, Zolfaghari R, Rosales FJ, Dietz J, Shimada T, et al. Regulation of hepatic vitamin A storage in a rat model of controlled vitamin A status during aging. J. Nutr. 2000;130:1280–1286
  16. Harrison EH. Lipases and carboxylases (possible roles in the hepatic metabolism of retinol). Annu. Rev. Nutr. 1998;18:259–276
  17. Gressner AM, Bachem MG. Molecular mechanisms of liver fibrogenesis-A homage to the role of activated fat-storing cells. Digestion. 1995;56:3335–3346
  18. Hautekeete ML, Geerts A. The hepatic stellate (Ito) cell (its role in human liver disease). Virchows Arch. 1997;430:195–207
  19. Knook DL, Blaner WS, Brouwer A, Hendriks HFJ. The role of non-parenchymal cells in liver retinoid metabolism. In:  Knook DL,  Wisse E,  Wake K editor. Cells of the Hepatic Sinusoids. vol II:Leiden: Kupffer Cell Foundation; 1989;p. 16–19
  20. Blomhoff R, Wake K. Perisinusoidal stellate cells of the liver (important roles in retinol metabolism and fibrosis). FASEB J. 1991;5:271–277
  21. Azaı̈s-Braesco V, Hautekeete ML, Dodeman I, Geerts A. Morphology of liver stellate cells and liver vitamin A content in 3,4,3′,4′,-tetrachlorobiphrnyl-treated rats. J. Hepatol. 1997;27:545–553
  22. Sato T, Kato R, Tyson C. Regulation of differentiated phenotype of rat hepatic lipocytes by retinoids in primary culture. Exp. Cell Res. 1995;271:72–83
  23. Matsuura T, Gad MZ, Harrison EH, Ross AC. Lecithin:retinol acyltransferase and retinyl ester hydrolase activities are differentially regulated by retinoids and have distinct distributions between hepatocytes and nonparenchymal cell fractions of rat liver. J. Nutr. 1997;127:218–224
  24. Nilsson A, Troen G, Petersen LB, Reppe L, Norum KR, Blomhoff R. Retinyl ester storage is altered in liver stellate cells and in HL60 cells transfected with cellular retinol-binding protein type I. Int. J. Biochem. Cell Biol. 1997;29:381–389
  25. Margis R, Borojevic R. Retinoid-mediated induction of the fat-storing phenotype in a liver connective tissue cell line (GRX). Biochim. Biophys. Acta. 1989;1011:1–5
  26. Vicente CV, Fortuna VA, Margis R, Trugo L, Borojevic R. Retinol uptake and metabolism, and cellular retinol binding protein expression in an in vitro model of hepatic stellate cell. Mol. Cell. Biochem. 1998;187:11–21
  27. Troen G, Nilsson A, Norum KR, Blomhoff R. Characterization of liver stellate cell retinyl ester storage. Biochem. J. 1994;300:793–798
  28. Borojevic R, Monteiro ANA, Vinhas SA, Domont GB, Mourão PAS, Emonard H, et al. Establishment of a continuous cell line from fibrotic schistosomal granulomas in mice livers. In Vitro Cell. Develop. Biol. 1985;21:382–390
  29. Borojevic R, Guaragna RG, Margis R, Dutra HS. In vitro induction of the fat-storing phenotype in a liver connective tissue cell line-GRX. In Vitro Cell. Develop. Biol. 1990;25:361–368
  30. Hendriks HFJ, Brouwer A, Knook DL. Isolation, purification, and characterization of liver cell types. Methods Enzymol. 1990;190:49–58
  31. Got L, Gousson T, Delacoux E. Simultaneous determination of retinyl esters and retinol in human livers by reversed-phase high-performance liquid chromatography. J. Chromatogr. B. 1995;668:233–239
  32. Markwell MAK, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem. 1978;87:206–210
  33. Ross AC. Retinol esterification by rat liver microsomes (evidence for a fatty acyl coenzyme A:retinol acyltransferase). J. Biol. Chem. 1982;257:2453–2459
  34. MacDonald PN, Ong DE. Assay of lecithin-retinol acyltransferase. Methods Enzymol. 1990;189:450–459
  35. Blaner WS, van Bennekum AM, Brouwer A, Hendriks HFJ. Distribution of lecithin-retinol acyltransferase activity in different types of rat liver cells and subcellular fractions. FEBS Lett. 1990;274:89–92
  36. Lichteinstein AH, Brecher P. Properties of Acyl-CoA (Cholesterol acyltransferase in rat liver microsomes). J. Biol. Chem. 1980;255:9098–9104
  37. Matsuura T, Ross AC. Regulation of hepatic lecithin-retinol acyltransferase activity by retinoic acid. Arch. Biochem. Biophys. 1993;301:221–227
  38. Shimada T, Ross AC, Muccio DD, Brouillette WJ, Shealy YF. Regulation of hepatic lecithin-retinol acyltransferase activity by retinoic acid receptor-selective retinoids. Arch. Biochem. Biophys. 1997;344:220–227
  39. Yost RW, Harrison EH, Ross AC. Esterification by rat liver microsomes of retinol bound to cellular retinol-binding protein. J. Biol. Chem. 1988;263:18693–18701
  40. Randolph RK, Ross AC. Vitamin A status regulates hepatic lecithin-retinol acyltransferase activity in rats. J. Biol. Chem. 1991;266:16453–16457
  41. Ghyselinck NB, Bavik C, Sapin V, Mark M, Bonnier D, Hindelang C, et al. Cellular retinol-binding protein I is essential for vitamin A homeostasis. EMBO J. 1999;18:4903–4914
  42. Williams IH, Polakis SE. Differentiation of 3T3-L1 fibroblasts to adipocytes. The effect of indomethacin, prostaglandin E1 and cyclic AMP on the process of differentiation. Biochem. Biophys. Res. Comm. 1977;77:175–186
  43. Guaragna RM, Trugo L, Borojevic R. Neutral lipid synthesis and accumulation during in vitro induction of the lipocyte phenotype in hepatic connective tissue cells. Biochim. Biophys. Acta. 1991;1085:29–34
  44. Guaragna RM, Trugo L, Borojevic R. Phospholipid modifications during conversion of hepatic myofibroblast into lipocytes (Ito-cells). Biochim. Biophys. Acta. 1992;1128:237–243
  45. Yumoto S, Ueno K, Mori S, Takebayashi N, Handa S. Morphological and biochemical analyses of lipid granules isolated from fat-storing cells in rat liver. Biomed. Res. 1988;9:147–160
  46. Randolph RK, Ross AC. Regulation of retinol uptake and esterification in MCF-7 and HepG2 cells by exogenous fatty acids. J. Lipid Res. 1991;32:809–820

PII: S0955-2863(01)00179-6

The Journal of Nutritional Biochemistry
Volume 12, Issue 11 , Pages 610-621 , November 2001